The player interacts with Ame exclusively through a pastel-themed Windows 95-esque user interface, selecting her daily tasks via desktop icons, monitoring her stats via the Task Manager, and conversing with her through an instant messenger service called JINE. Each day is divided into noon, dusk, and evening timeslots, and various actions can take up one or multiple of these timeslots. Ame can only livestream during the evening, as that is the time of day where stream viewers are most active. During Ame's livestreams, the player takes on the role of a content moderator responsible for deleting or promoting viewer comments within the stream's livechat. After streaming or concluding various activities, the player is able to obtain a glimpse of Ame's thoughts and mood via her posts on Tweeter.

Alongside follower count, Ame has three stats which the player will need to monitor, namely stress, affection (towards the player), and mental darkness. Should certain attributes become too high or low, Ame will begin to display adverse effects. Activities that the player can choose for Ame may involve searching for new stream ideas, spending time together, sleeping, or abusing prescription and illicit drugs. Each of these activities affects Ame's stats in differing ways.

At release, there were originally 22 different endings to the game, which are encountered based on the player's choices. Additional endings were added in an October 28, 2022 update coinciding with the game's Nintendo Switch release.

In-game screenshot, demonstrating the game interface. A live feed of Ame can be seen in the top-left, while her stats can be monitored in the Task Manager to the top-right, and the player converses with her using the JINE messenger on the bottom-right. Daily activities are selected via the desktop icons.